OsVTC1-1 RNAi Mutant with Reduction of Ascorbic Acid Synthesis Alters Cell Wall Sugar Composition and Cell Wall-Associated Proteins
نویسندگان
چکیده
Ascorbic acid (AsA) or Vitamin C is an antioxidant molecule and plays important role in many biological processes plants. GDP-D-mannose pyrophosphorylase (GMP VTC1) catalyzes the synthesis of GDP-D-mannose, which a precursor for AsA production used cell wall polysaccharide glycoprotein synthesis. In rice, OsVTC1 gene consists three homologs, including OsVTC1-1, OsVTC1-3 OsVTC1-8. this study, we characterized wild type (WT) OsVTC1-1 RNAi lines (RI1-2 RI1-3) showed that transcript levels most genes pathway, content leaf anatomical parameters were reduced, revealing involved To further study gene, monosaccharide composition, transcriptome proteome compared, with specific attention paid to their lines. Mannose galactose composition (mole%) decreased Additionally, reduction wall-associated proteins, such as kinesin, expansin, beta-galactosidase cellulose synthase observed Our results suggest wall-related processes.
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ژورنال
عنوان ژورنال: Agronomy
سال: 2022
ISSN: ['2156-3276', '0065-4663']
DOI: https://doi.org/10.3390/agronomy12061272